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TET2 antibody [GT411]

Anti-TET2 antibody [GT411] used in Western Blot (WB). GTX629879
Anti-TET2 antibody [GT411] used in Immunoprecipitation (IP). GTX629879

Cat. No. GTX629879

Host

Mouse

Clonality

Monoclonal

Clone Name

GT411

Isotype

IgG1

Application

WB, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

DDDDK-human TET2-transfected 293T cells

Product Note

Knockdown/Knockout validation was supported by customer review data.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888169

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 2 , KIAA1546 , MDS

Database

Research Area

DATA IMAGES

Anti-TET2 antibody [GT411] used in Western Blot (WB). GTX629879

GTX629879 WB Image

TET2 antibody [GT411] detects TET2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
5% SDS-PAGE
TET2 antibody [GT411] (GTX629879) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TET2 antibody [GT411] used in Immunoprecipitation (IP). GTX629879

GTX629879 IP Image

TET2 antibody [GT411] immunoprecipitates TET2 protein in IP experiments.
IP samples: 30 μg whole cell extract of TET2-transfected 293T cells.
A. Control with 5 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TET2 protein by 5 μg TET2 antibody [GT411] (GTX629879)
5 % SDS-PAGE
The immunoprecipitated TET2 protein was detected by TET2 antibody [GT411] (GTX629879) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

There are currently no references for TET2 antibody [GT411] (GTX629879). Be the first to share your publications with this product.

REVIEW

TET2 antibody [GT411] Cat. No. GTX629879

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 06-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : M10 cells
Amount used : 20μg
Blocking : 10% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Lane Description : Lane 1: M10 cells, Lane 2: Knockdown of human TET2 in M10 cells
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations