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TIGIT antibody [1G9] (APC)

Anti-TIGIT antibody [1G9] (APC) used in Flow cytometry (FACS). GTX01496-07

Cat. No. GTX01496-07

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1G9

Isotype

 IgG1

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($249)
See all TIGIT products
PRODUCT

Summary

The 1G9 antibody reacts with mouse TIGIT (T cell Ig and ITIM domain), a 26 kDa member of the CD28 receptor family which is reported to regulate T cell receptor (TCR) activation. Within the CD28 family of receptors there are those which have co-stimulatory activity, such as CD28 and CTLA-4, as well as more recently identified receptors like TIGIT which are proposed to provide co-inhibitory signals. TIGIT is expressed and upregulated on activated T cells, and is also expressed on memory and regulatory T cells. Upon engagement by its ligands, CD112 and CD155, TIGIT signaling inhibits T cell proliferation and suppresses T cell responses, without triggering cell deletion. A second inhibitory effect of TIGIT signaling is the generation of immunoregulatory dendritic cells, which secrete IL-10 and TGF-beta to further inhibit T cell function.The 1G9 antibody may be used for flow cytometric analysis of TIGIT, which is expressed at very high levels on T regulatory cells (Tregs) and activated conventional T cells, as well as memory T cells and NK cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-TIGIT antibody [1G9] (APC) used in Flow cytometry (FACS). GTX01496-07

GTX01496-07 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01496-07 TIGIT antibody [1G9] (APC).
Right panel : co-stained with TIGIT antibody [1G9] (APC) and Mouse CD4 antibody (FITC)
Left panel : co-stained with isotype control and Mouse CD4 antibody (FITC)
antibody amount : 0.06 μg (5 μl)

REFERENCE

Clone Reference

2012; Immune checkpoints in central nervous system autoimmunity.
2011; Cutting edge: TIGIT has T cell-intrinsic inhibitory functions.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 249