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TIM-1 antibody

KO/KD
Anti-TIM-1 antibody used in Western Blot (WB). GTX85067
Anti-TIM-1 antibody used in Western Blot (WB). GTX85067
Anti-TIM-1 antibody used in Western Blot (WB). GTX85067
Anti-TIM-1 antibody used in Western Blot (WB). GTX85067

Cat. No. GTX85067

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TIM-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Human Uterus Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TIM-1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-1.The immunogen is located within amino acids 230 - 280 of TIM-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721944

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatitis A virus cellular receptor 1 , CD365 , HAVCR , HAVCR-1 , KIM-1 , KIM1 , TIM , TIM-1 , TIM1 , TIMD-1 , TIMD1

Cellular Localization

Membrane

Background

The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

Database

Research Area

DATA IMAGES

Anti-TIM-1 antibody used in Western Blot (WB). GTX85067

GTX85067 WB Image

WB analysis of various sample lysates using GTX85067 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 8 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85067

GTX85067 WB Image

WB analysis of various sample lysates using GTX85067 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85067

GTX85067 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) using GTX85067 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85067

GTX85067 WB Image

WB analysis of human uterus tissue lysate using GTX85067 TIM-1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

Application Reference

AS Arellano Buendia et al. PeerJ 2023; Antioxidant and anti-inflammatory effects of allicin in the kidney of an experimental model of metabolic syndrome
Prieto-Carrasco R et al. Biology (Basel) 2021; 10 (5) Progressive Reduction in Mitochondrial Mass Is Triggered by Alterations in Mitochondrial Biogenesis and Dynamics in Chronic Kidney Disease Induced by 5/6 Nephrectomy.
Application : WB
Garc?a-Arroyo FE et al. Int J Mol Sci 2021; 22 (5) Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet.
Application : WB
Reactivity : Mouse
Fernando E Garc?a-Arroyo et al. Oxid Med Cell Longev 2020; 2020 : 8850266 Fluid Intake Restriction Concomitant to Sweetened Beverages Hydration Induce Kidney Damage.
Application : WB
Reactivity : Rat
Garc?a-Arroyo FE et al. Biomed Res Int 2020; 2020 : 4281802 Restricted Water Intake and Hydration with Fructose-Containing Beverages during Infancy Predispose to Aggravate an Acute Renal Ischemic Insult in Adolescent Rats.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399