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TIM-3 antibody

Anti-TIM-3 antibody used in Western Blot (WB). GTX65883
Anti-TIM-3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65883

Cat. No. GTX65883

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all TIM-3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HAVCR2 (NP_116171.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatitis A virus cellular receptor 2 , CD366 , HAVcr-2 , KIM-3 , TIM3 , TIMD-3 , TIMD3 , Tim-3

Cellular Localization

Membrane,Cell junction

Background

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-TIM-3 antibody used in Western Blot (WB). GTX65883

GTX65883 WB Image

WB analysis of various sample lysates using GTX65883 TIM-3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TIM-3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65883

GTX65883 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX65883 TIM-3 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399