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TIP-5 antibody - ChIP grade

Anti-TIP-5 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60851
Anti-TIP-5 antibody - ChIP grade used in Western Blot (WB). GTX60851

Cat. No. GTX60851

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ChIP assay

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all TIP-5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ChIP assay 1-10 μl
Not tested in other applications.

Calculated MW

211 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

TIP5 (Transcription termination factor I-interacting protein 5), using the recombinant protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bromodomain adjacent to zinc finger domain 2A , TIP5 , WALp3

Cellular Localization

Nucleolus

Database

Research Area

DATA IMAGES

Anti-TIP-5 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60851

GTX60851 ChIP assay Image

ChIP analysis of HEK293T and NIH3T3 cells using GTX60851 TIP-5 antibody - ChIP grade. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4˚C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP'd DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. This figure shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

Anti-TIP-5 antibody - ChIP grade used in Western Blot (WB). GTX60851

GTX60851 WB Image

WB analysis of 150 μg nuclear extract from either NIH3T3 or HeLa cells using GTX60851 TIP-5 antibody - ChIP grade.
Dilution : 1:1,000

REFERENCE

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Package List Price ($)
$ 399