APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Daudi Cell Lysate(GTX23951) , Rat Liver Tissue Lysate(GTX27943)
Product Note
This antibody is predicted to recognize TIP47 isoforms 1-4 (UniProt #O60664).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TIP47 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TIP47. The immunogen is located within aa 385-434 of human TIP47 (UniProt #O60664).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
perilipin 3 , M6PRBP1 , PP17 , TIP47
Cellular Localization
Cytoplasm,Endosome membrane
Background
Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
Database
Research Area
DATA IMAGES
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GTX85492 IHC-P Image
IHC-P analysis of rat liver tissue using GTX85492 TIP47 antibody.
Working concentration : 20 μg/ml
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GTX85492 IHC-P Image
IHC-P analysis of rat liver tissue using GTX85492 TIP47 antibody.
Working concentration : 10 μg/ml
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GTX85492 WB Image
WB analysis of Daudi cell lysate using GTX85492 TIP47 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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REFERENCE
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REVIEW
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