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TLR4 antibody

Anti-TLR4 antibody used in Flow cytometry (FACS). GTX13556
Anti-TLR4 antibody used in Western Blot (WB). GTX13556
Anti-TLR4 antibody used in Flow cytometry (FACS). GTX13556
Anti-TLR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13556

Cat. No. GTX13556

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse
Package
100 μg ($419)
See all TLR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
ICC/IF 1:50
IHC-P 1:50
IHC-Fr 1:10 - 1:500
FACS 1 μg / 10⁶ cells
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was developed against a sythetic peptide corresponding to amino acids 420-456 of human TLR4.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll-like receptor 4 , Lps , Ly87 , Ran/M1 , Rasl2-8

Cellular Localization

Cell membrane

Background

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Database

Research Area

DATA IMAGES

Anti-TLR4 antibody used in Flow cytometry (FACS). GTX13556

GTX13556 FACS Image

FACS analysis of RAW264.7 cells using GTX13556 TLR4 antibody.
Red : Primary antibody
Green : isotype control
Antibody amount : 2μg

Anti-TLR4 antibody used in Western Blot (WB). GTX13556

GTX13556 WB Image

WB analysis of recombinant mouse TLR4 (partial) protein using GTX13556 TLR4 antibody.
Dilution : 2 μg/ml

Anti-TLR4 antibody used in Flow cytometry (FACS). GTX13556

GTX13556 FACS Image

FACS analysis of mouse macrophage cells using GTX13556 TLR4 antibody.
Red : primary antibody
Green : isotype control
Antibody amount : 1μg

Anti-TLR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13556

GTX13556 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX13556 TLR4 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:50
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419