APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1μg/ml |
| 5μg/ml |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide corresponding to aa 182-195 of human ASC.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ASC , CARD5 , PYCARD , PYD and CARD domain containing , TMS , TMS1
Cellular Localization
Cytoplasm,Endoplasmic reticulum,Mitochondrion,Nucleus
Background
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX28394 IHC-P Image
IHC-P analysis of 4% PFA-fixed Human Spleen Tissue using GTX28394 TMS1 antibody.
Dilution : 20μg/mL
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GTX28394 ICC/IF Image
ICC/IF analysis of formaldehyde-fixed HL-60 cells using GTX28394 TMS1 antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5μg/mL
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GTX28394 IHC-P Image
IHC-P analysis of formaldehyde fixed Human Spleen Tissue using GTX28394 TMS1 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL
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GTX28394 WB Image
WB analysis of HL-60 cell lysate using GTX28394 TMS1 antibody in the absence (A) or presence (B) of blocking peptide.
Dilution : 1μg/mL
Loading : 15μg of lysates per lane
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GTX28394 WB Image
WB analysis of THP-1 cell lysate using GTX28394 TMS1 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane
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GTX28394 WB Image
WB analysis of various samples using GTX28394 TMS1 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane
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REFERENCE
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REVIEW
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