GeneTex
United States (US)

TNF alpha recombinant protein

Cat No. GTX65409

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Rat
Species Rat
APPLICATION

Application Note

The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2x10^7 units/mg.

Calculated MW

17.0 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

1X PBS

Storage

We recommend a quick spin followed by reconstitution in water to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Rat

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Necrosis Factor,Rattnf,Tnf-Alpha,Tnfa,Tnf

Background

Tumor Necrosis Factor-alpha (TNF-alpha ) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-alpha is a 17 kDa protein containing 157 amino acid residues.

Research Area