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TOM70 antibody

Orthogonal
Anti-TOM70 antibody used in IHC (Paraffin sections) (IHC-P). GTX85353
Anti-TOM70 antibody used in Western Blot (WB). GTX85353
Anti-TOM70 antibody used in IHC (Paraffin sections) (IHC-P). GTX85353
Anti-TOM70 antibody used in Western Blot (WB). GTX85353

Cat. No. GTX85353

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TOM70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

Product Note

This antibody is specific for TOM70 Internal

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TOM70 antibody was raised against a 15 amino acid synthetic peptide near the center of human TOM70.The immunogen is located within amino acids 220 - 270 of TOM70.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726634

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocase of outer mitochondrial membrane 70 , TOMM70A , Tom70

Cellular Localization

Mitochondrion outer membrane

Background

The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear. At least two isoforms of TOM70 are known to exist.

Database

Research Area

DATA IMAGES

Anti-TOM70 antibody used in IHC (Paraffin sections) (IHC-P). GTX85353

GTX85353 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85353 TOM70 antibody.
Working concentration : 2.5 μg/ml

Anti-TOM70 antibody used in Western Blot (WB). GTX85353

GTX85353 WB Image

WB analysis of rat brain tissue lysate using GTX85353 TOM70 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-TOM70 antibody used in IHC (Paraffin sections) (IHC-P). GTX85353

GTX85353 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85353 TOM70 antibody.
Working concentration : 20 μg/ml

Anti-TOM70 antibody used in Western Blot (WB). GTX85353

GTX85353 WB Image

Orthogonal

The data was published in the journal PLoS Pathog in 2015. PMID: 25816318

REFERENCE

Application Reference

Kao YT et al. PLoS Pathog 2015; 11 (3) : e1004750 Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid β-oxidation.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399