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TOMM40 antibody

Orthogonal
Anti-TOMM40 antibody used in Western Blot (WB). GTX133780
Anti-TOMM40 antibody used in IHC (Paraffin sections) (IHC-P). GTX133780
Anti-TOMM40 antibody used in Western Blot (WB). GTX133780
Anti-TOMM40 antibody used in IHC (Paraffin sections) (IHC-P). GTX133780

Cat. No. GTX133780

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TOMM40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TOMM40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887107

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocase of outer mitochondrial membrane 40 , C19orf1 , D19S1177E , PER-EC1 , PEREC1 , TOM40

Cellular Localization

Mitochondrion outer membrane; Multi-pass membrane protein

Background

TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria (Humphries et al., 2005 [PubMed 15644312]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TOMM40 antibody used in Western Blot (WB). GTX133780

GTX133780 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with TOMM40 antibody (GTX133780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM40 antibody used in IHC (Paraffin sections) (IHC-P). GTX133780

GTX133780 IHC-P Image

TOMM40 antibody detects TOMM40 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TOMM40 stained by TOMM40 antibody (GTX133780) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM40 antibody used in Western Blot (WB). GTX133780

GTX133780 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOMM40 antibody (GTX133780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM40 antibody used in IHC (Paraffin sections) (IHC-P). GTX133780

GTX133780 IHC-P Image

TOMM40 antibody detects TOMM40 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
TOMM40 stained by TOMM40 antibody (GTX133780) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Avolio R et al. Genome Res 2023; 33 (8) : 1242-1257 Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Avolio R et al. bioRxiv 2023; Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteins.
Application : WB, PLA
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169