GeneTex
United States (US)

TRAIL protein

Cat No. GTX29960

Application in vitro
Reactivity Human
Species Human
APPLICATION

Application Note

The activity is determined by the cytolysis of Murine L929 cells in the presence of Actinomycin D, ED50 for this effect is less than 2ng/ml, corresponding to a specific activity of 5,000,000IU/mg.
PROPERTIES

Form

Lyophilized powder

Buffer

20mM Tris-HCl (pH 8.0) and 150mM NaCl

Storage

Lyophilized powder is stable at room temperature for 3 weeks, should be stored below -18ºC for long term storage. It is recommended to reconstitute the lyophilized powder in sterile H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution, protein reagent should be stored at 4ºC between 2-7 days and for future use below -18ºC. For extended storage, add 0.1% HSA or BSA, aliquot and store at -18ºC or below. Avoid freeze-thaw cycles.

Antigen Species

Human

Immunogen

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

Region/Sequence

MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG

Expression System

E. coli

Purification

Purified

Purity

Greater than 95% by SDS-PAGE and HPLC analyses.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cell Membrane

Background

Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues. TRAIL is a type II membrane protein from the TNF family. The human form of the protein is 281 amino acids in length, whereas the murine form is 291 amino acids. The two forms share 65% identity.
GTX29960 TRAIL protein contains 168 amino acids and has a molecular mass of approximately 21 kDa.

Research Area

Package List Price ($)
$ 299