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TRIB3 antibody [N3C3]

Orthogonal
Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991
Anti-TRIB3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113991
Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991
Anti-TRIB3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113991

Cat. No. GTX113991

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRIB3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HUVEC

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038166

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tribbles pseudokinase 3 , C20orf97 , NIPK , SINK , SKIP3 , TRB3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991

GTX113991 WB Image

Sample (15 μg of whole cell lysate)
A: HUVEC
GTX113991 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIB3 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113991

GTX113991 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TRB-3 (GTX113991) antibody at 1:200 dilution.

Anti-TRIB3 antibody [N3C3] used in Western Blot (WB). GTX113991

GTX113991 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIB3 antibody [N3C3] (GTX113991) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TRIB3 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX113991

GTX113991 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using TRB-3 (GTX113991) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lin S et al. Int J Biochem Cell Biol 2017; 87 : 54-68 endoplasmic reticulum-targeting photosensitizer hypericin confers chemo-sensitization towards * through inducing pro-death autophagy.
Application : WB
Reactivity : Human
Hsieh YY et al. Am J Cancer Res 2016; 6 (8) : 1661-80 EZH2 inhibitors transcriptionally upregulate cytotoxic autophagy and cytoprotective unfolded protein response in human colorectal cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169