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TRIM25 antibody

Anti-TRIM25 antibody used in Immunoprecipitation (IP). GTX116209
Anti-TRIM25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116209
Anti-TRIM25 antibody used in IHC (Paraffin sections) (IHC-P). GTX116209
Anti-TRIM25 antibody used in Western Blot (WB). GTX116209
Anti-TRIM25 antibody used in Western Blot (WB). GTX116209

Cat. No. GTX116209

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRIM25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A549 , Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175569

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 25 , EFP , RNF147 , Z147 , ZNF147

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIM25 antibody used in Immunoprecipitation (IP). GTX116209

GTX116209 IP Image

TRIM25 antibody immunoprecipitates TRIM25 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TRIM25 protein by 2 μg of TRIM25 antibody (GTX116209) 7.5% SDS-PAGE The immunoprecipitated TRIM25 protein was detected by TRIM25 antibody (GTX116209) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TRIM25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116209

GTX116209 ICC/IF Image

TRIM25 antibody detects TRIM25 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: TRIM25 protein stained by TRIM25 antibody (GTX116209) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TRIM25 antibody used in IHC (Paraffin sections) (IHC-P). GTX116209

GTX116209 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRIM25(GTX116209) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRIM25 antibody used in Western Blot (WB). GTX116209

GTX116209 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody (GTX116209) diluted at 1:500.

Anti-TRIM25 antibody used in Western Blot (WB). GTX116209

GTX116209 WB Image

Sample (30 ug of whole cell lysate)

A: A549

7.5% SDS PAGE

GTX116209 diluted at 1:1000

REFERENCE

Application Reference

Okudera M et al. Neuroimmunomodulation 2021; : 44571 Expression of Zinc-Finger Antiviral Protein in hCMEC/D3 Human Cerebral Microvascular Endothelial Cells: Effect of a Toll-Like Receptor 3 Agonist.
El-Asmi F et al. Cytokine 2020; 129 : 155025 Cross-talk between SUMOylation and ISGylation in response to interferon.
Application : WB
Reactivity : Human
Qin X et al. Biochem Biophys Res Commun 2017; (Epub) trim25 is associated with * resistance in non-small-cell lung carcinoma a549-cell line via downregulation of 14-3-3σ.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169