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TRIM25 antibody

Anti-TRIM25 antibody used in Western Blot (WB). GTX31323
Anti-TRIM25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31323
Anti-TRIM25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31323

Cat. No. GTX31323

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all TRIM25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TRIM25 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TRIM25.The immunogen is located within amino acids 520 - 570 of TRIM25.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 25 , EFP , RNF147 , Z147 , ZNF147

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRIM25 antibody used in Western Blot (WB). GTX31323

GTX31323 WB Image

WB analysis of HeLa cell lysate in (A) the absence and (B) presence of blocking peptide using GTX31323 TRIM25 antibody.
Working concentration : 1 μg/ml

Anti-TRIM25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31323

GTX31323 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31323 TRIM25 antibody.
Working concentration : 20 μg/ml

Anti-TRIM25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31323

GTX31323 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31323 TRIM25 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399