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TRIM3 antibody, N-term

Anti-TRIM3 antibody, N-term used in Western Blot (WB). GTX89826
Anti-TRIM3 antibody, N-term used in Western Blot (WB). GTX89826

Cat. No. GTX89826

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TRIM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89826-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2; NP_150594.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721685

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 3 , BERP , HAC1 , RNF22 , RNF97

Cellular Localization

Cytoplasm , Cell projection, dendrite

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRIM3 antibody, N-term used in Western Blot (WB). GTX89826

GTX89826 WB Image

WB analysis of mouse brain lysate using GTX89826 TRIM3 antibody, N-term.
Dilution : 0.05μg/ml
Loading : 30μg protein in RIPA buffer

Anti-TRIM3 antibody, N-term used in Western Blot (WB). GTX89826

GTX89826 WB Image

WB analysis of human cerebellum (A) and rat (B) brain lysate using GTX89826 TRIM3 antibody, N-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Chen G et al. Cancer Res 2014; Human Brat ortholog TRIM3 is a tumor suppressor that regulates asymmetric cell division in glioblastoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399