TRIM5 antibody, N-term

Cat. No. GTX47603

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX47603 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	2-6μg/ml
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX47603-PEP).

Product Note

This antibody is expected to recognise all three human isoforms of this protein.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1; NP_149083.1; NP_149084.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 5 , RNF88 , TRIM5alpha

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

Database

Gene ID: 85363 TRIM5
UniProt: Q9C035 TRIM5

Research Area

DATA IMAGES

GTX47603 WB Image

WB analysis of human thymus lysate (35μg protein in RIPA buffer) using GTX47603 TRIM5 antibody, N-term with (Lane B) and without (Lane A) blocking with the immunizing peptide.
Dilution : 1μg/ml
with (Lane B) and without (Lane A) blocking with the immunizing peptide.
Dilution : 1μg/ml

GTX47603 IHC-P Image

IHC-P analysis of human colon tissue using GTX47603 TRIM5 antibody, N-term.
Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 3μg/ml

Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 3μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty