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TRPA1 antibody

Orthogonal
Anti-TRPA1 antibody used in Live cell imaging (LCI). GTX54765
Anti-TRPA1 antibody used in Immunoprecipitation (IP). GTX54765
Anti-TRPA1 antibody used in Western Blot (WB). GTX54765
Anti-TRPA1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54765

Cat. No. GTX54765

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP, LCI

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 (1st  extracellular loop) of human TRPA1 (Accession  : O75762).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887885

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily A member 1 , ANKTM1 , FEPS , FEPS1

Cellular Localization

Cell membrane

Background

The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRPA1 antibody used in Live cell imaging (LCI). GTX54765

GTX54765 LCI Image

Live cell imaging analysis of intact living rat PC12 cells using GTX54765 TRPA1 antibody.
Panel A : Primary antibody (red)
Panel B : Live view of the cell
Panel C : Merged images of Panel A and B
Dilution : 1:50

Anti-TRPA1 antibody used in Immunoprecipitation (IP). GTX54765

GTX54765 IP Image

IP analysis of PC-12 cell lysate using GTX54765 TRPA1 antibody.
IP reaction : 6.5 μg antibody
Lane 1 : PC-12 lysates
Lane 2 : PC-12 lysates + Anti-TRPA1 Antibody + protein A beads
Lane 3 : Anti-TRPA1 Antibody + protein A beads

Anti-TRPA1 antibody used in Western Blot (WB). GTX54765

GTX54765 WB Image

Orthogonal

WB analysis of rat DRG (lanes 1,2), non-differentiated PC12  cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates using GTX54765 TRPA1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPA1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54765

GTX54765 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54765 TRPA1 antibody.
Panel A : TRPA1 (green) is detected in both cells (horizontal arrow) and processes (vertical arrow).
Panel B : Nuclear staining using DAPI as the counterstain.
Panel C : Merged image of A and B.
Dilution : 1:200

REFERENCE

Application Reference

Hao M et al. Front Cell Infect Microbiol 2023; 13 : 1105272 Improvement of loperamide-hydrochloride-induced intestinal motility disturbance by Platycodon grandiflorum polysaccharides through effects on gut microbes and colonic serotonin.
Application : WB
Reactivity : Rat
Jiang YH et al. Int J Mol Sci 2023; 24 (1) Sensory Receptor, Inflammatory, and Apoptotic Protein Expression in the Bladder Urothelium of Patients with Different Subtypes of Interstitial Cystitis/Bladder Pain Syndrome.
Application : IHC-Fr
Reactivity : Human
Liu X et al. Mol Pain 2023; 19 : 1.74481E+16 Toll-like receptor 4 signaling pathway in sensory neurons mediates remifentanil-induced postoperative hyperalgesia via transient receptor potential ankyrin 1.
Application : WB, IHC-Fr
Reactivity : Rat
Sung Youl Kim et al. Int J Mol Sci 2022; 23 (9) Mesenchymal Stem Cells and Extracellular Vesicles Derived from Canine Adipose Tissue Ameliorates Inflammation, Skin Barrier Function and Pruritus by Reducing JAK/STAT Signaling in Atopic Dermatitis.
Umehara Y et al. Sci Rep 2020; 10 (1) : 4360 Robust induction of neural crest cells to derive peripheral sensory neurons from human induced pluripotent stem cells.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639