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TRPC3 antibody

Anti-TRPC3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54860
Anti-TRPC3 antibody used in Western Blot (WB). GTX54860
Anti-TRPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54860
Anti-TRPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54860
Anti-TRPC3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54860
Anti-TRPC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54860

Cat. No. GTX54860

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 (Intracellular, C-terminus) of mouse TRPC3 (Accession  : Q9QZC1).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887899

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel, subfamily C, member 3 , Mwk , Trcp3 , Trp3 , Trrp3

Database

Research Area

DATA IMAGES

Anti-TRPC3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54860

GTX54860 IHC-Fr Image

IHC-Fr analysis of rat cerebellum tissue using GTX54860 TRPC3 antibody. DAPI is used as the counterstain (blue).
Panel A : TRPC3 (green) appears in Purkinje cells (arrows) including both soma and dendrites and as well as in the molecular layer neuropil (asterisk). Staining of the same section with mouse anti-parvalbumin (red) reveals that TRPC3 is not expressed in molecular layer interneurons.
Panel B : Pre-incubating Anti-TRPC3 with the peptide antigen

Anti-TRPC3 antibody used in Western Blot (WB). GTX54860

GTX54860 WB Image

WB analysis of rat heart membrane lysate using GTX54860 TRPC3 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54860

GTX54860 IHC-P Image

IHC-P analysis of rat pituitary gland tissue using GTX54860 TRPC3 antibody. TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
Dilution : 1:100

Anti-TRPC3 antibody used in IHC (Paraffin sections) (IHC-P). GTX54860

GTX54860 IHC-P Image

IHC-P analysis of mouse cerebellum tissue using GTX54860 TRPC3 antibody.
Panel A : Primary antibody
Panel B : Antibody preincubated with the control peptide antigen

Anti-TRPC3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54860

GTX54860 IHC-Fr Image

IHC-Fr analysis of rat cerebellum tissue using GTX54860 TRPC3 antibody. DAPI is used as the counterstain (blue).
Panel A : TRPC6 (green) appears in molecular layer and in Purkinje cells.
Panel B : In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells.
Panel C : Merge images of A and B indicates extensive co-localization.

Anti-TRPC3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54860

GTX54860 ICC/IF Image

ICC/IF analysis of PFA-fixed C6 cells using GTX54860 TRPC3 antibody.
Panel A : Primary antibody
Panel B : DNA dye Hoechst 33342
Panel C : Merged images of panels A and B
Dilution : 1:500

REFERENCE

Application Reference

Ge P et al. Cell Biol Int 2018; (Epub) TRPC1/3/6 inhibition attenuates the TGF-β1-induced epithelial-mesenchymal transition in gastric cancer via the Ras/Raf1/ERK signaling pathway.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639