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TRPC5 antibody

Anti-TRPC5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54861
Anti-TRPC5 antibody used in Western Blot (WB). GTX54861

Cat. No. GTX54861

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

111 kDa. ( Note )

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 (Intracellular, C-terminus) of human TRPC5 (Accession Q9UL62).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887900

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily C member 5 , PPP1R159 , TRP5

Cellular Localization

Cell membrane

Background

This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-TRPC5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54861

GTX54861 IHC-Fr Image

IHC-Fr analysis of rat cortex tissue using GTX54861 TRPC5 antibody.
Panel A : TRPC5 (green) appears in pyramidal shaped neuronal soma (vertical arrows) and apical dendrites (horizontal arrows).
Panel B : Nuclear staining using DAPI (blue).
Panel C : Merged image of A and B.

Anti-TRPC5 antibody used in Western Blot (WB). GTX54861

GTX54861 WB Image

WB analysis of rat brain membrane lysate using GTX54861 TRPC5 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

Application Reference

Wei L et al. Cell Physiol Biochem 2017; 44 (6) : 2228-2242 Rosiglitazone Inhibits Angiotensin II-Induced Proliferation of Glomerular Mesangial Cells via the Gαq/Plcβ4/TRPC Signaling Pathway.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639