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TRPM4 antibody [10H5]

Overexpression
Anti-TRPM4 antibody [10H5] used in Western Blot (WB). GTX83489
Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489
Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489
Anti-TRPM4 antibody [10H5] used in Western Blot (WB). GTX83489
Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489
Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489
Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489

Cat. No. GTX83489

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10H5

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($539)
See all TRPM4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel subfamily M member 4 , LTrpC4 , PFHB1B , TRPM4B , hTRPM4

Cellular Localization

Endoplasmic reticulum,Golgi apparatus

Background

Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.

Database

Research Area

DATA IMAGES

Anti-TRPM4 antibody [10H5] used in Western Blot (WB). GTX83489

GTX83489 WB Image

Overexpression

WB analysis of HEK293T cells transfected with TRPM4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83489 TRPM4 antibody [10H5].
Loading : 5 ug per lane

Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489

GTX83489 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83489 TRPM4 antibody [10H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489

GTX83489 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83489 TRPM4 antibody [10H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TRPM4 antibody [10H5] used in Western Blot (WB). GTX83489

GTX83489 WB Image

WB analysis of various cell lines using GTX83489 TRPM4 antibody [10H5].
Loading : 35 ug per lane

Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489

GTX83489 IHC-P Image

IHC-P analysis of human liver tissue using GTX83489 TRPM4 antibody [10H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489

GTX83489 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX83489 TRPM4 antibody [10H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TRPM4 antibody [10H5] used in IHC (Paraffin sections) (IHC-P). GTX83489

GTX83489 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83489 TRPM4 antibody [10H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539