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Tau antibody

Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49353
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49353
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49353
Anti-Tau antibody used in Western Blot (WB). GTX49353
Anti-Tau antibody used in IHC (Frozen sections) (IHC-Fr). GTX49353
Anti-Tau antibody used in IHC (Frozen sections) (IHC-Fr). GTX49353

Cat No. GTX49353

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse, Rat
Package
50 μl ($289)
APPLICATION

Application Note

Western Blot: 1:10000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000.

Calculated MW

79 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Liquid with phosphate buffered saline plus 10mM sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant protein structure from E. coli. corresponding to the shortest version of the 8 human tau proteins, so the antibody is expected to bind to all 8 isoforms.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein Tau,Ddpac,Ftdp-17,Maptl,Mstd,Mtbt1,Mtbt2,Ppnd,Ppp1R103,Tau,Mapt

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49353

GTX49353 ICC/IF Image

Tau antibody detects Tau protein at by immunofluorescent analysis.
Sample: Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau protein stained by Tau antibody (GTX49353) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49353

GTX49353 ICC/IF Image

Tau antibody detects Tau protein at axon by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau protein stained by Tau antibody (GTX49353) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49353

GTX49353 ICC/IF Image

E18 hippocampal neurons stained with Tau (red) and Doublecortin (green). The two proteins overlap in the proximal dendrites (yellow) Axons (low doublecortin content) are red. Blue staining is the nuclear DNA.

Anti-Tau antibody used in Western Blot (WB). GTX49353

GTX49353 WB Image

Blots of crude rat brain extract. Tau protein is expressed as up to 8 different isoforms of different molecular weight and so appears as multiple closely spaced bands covering the region of the blot from 50kDa to 70kDa. Lane 11 was probed with CPCA-Tau while lane 12 was probed with tau monoclonal antibody.

Anti-Tau antibody used in IHC (Frozen sections) (IHC-Fr). GTX49353

GTX49353 IHC-Fr Image

Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Tau protein stained by Tau antibody (GTX49353) diluted at 1:250.
Red: MAP2, stained by MAP2 antibody (GTX133110) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Tau antibody used in IHC (Frozen sections) (IHC-Fr). GTX49353

GTX49353 IHC-Fr Image

Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Tau protein stained by Tau antibody (GTX49353) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
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Package List Price ($)
$ 289