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Thrombospondin 1 antibody

Anti-Thrombospondin 1 antibody used in Western Blot (WB). GTX130967
Anti-Thrombospondin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130967

Cat. No. GTX130967

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Thrombospondin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

Observed MW

180 kDa. The observed M.W. is based on the following publication. PMID: 17596205

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Thrombospondin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886396

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombospondin 1 , THBS , THBS-1 , TSP , TSP-1 , TSP1

Background

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Thrombospondin 1 antibody used in Western Blot (WB). GTX130967

GTX130967 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 1 antibody (GTX130967) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombospondin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130967

GTX130967 ICC/IF Image

Thrombospondin 1 antibody detects Thrombospondin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombospondin 1 protein stained by Thrombospondin 1 antibody (GTX130967) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Shih HJ et al. Am J Cancer Res 2020; 10 (6) : 1728-1744 IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169