APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:200 |
| 1:10-1:200 |
| 1:20-1:100 |
| Assay dependent |
| 1:10 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody does not detect TRv alpha -2 or TR beta-1.
PROPERTIES
Form
Liquid
Buffer
Serum diluted with PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Synthetic Peptide: E(403) V F E D Q E V(410)
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_380602
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thyroid hormone receptor alpha , AR7 , CHNG6 , EAR7 , ERB-T-1 , ERBA , ERBA1 , NR1A1 , THRA1 , THRA2 , c-ERBA-1
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX25621 IHC-P Image
IHC-P analysis of mouse thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX25621 IHC-P Image
IHC-P analysis of human thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:500
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GTX25621 ICC/IF Image
ICC/IF analysis of L6 cells using GTX25621 Thyroid Hormone Receptor alpha antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX25621 IHC-P Image
IHC-P analysis of human skin tissue using GTX25621 Thyroid Hormone Receptor alpha antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
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GTX25621 ICC/IF Image
ICC/IF analysis of A431 cells using GTX25621 Thyroid Hormone Receptor alpha antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX25621 IHC-P Image
IHC-P analysis of human thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20
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GTX25621 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX25621 Thyroid Hormone Receptor alpha antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:500
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GTX25621 IHC-P Image
IHC-P analysis of rat thyroid tissue using GTX25621 Thyroid Hormone Receptor alpha antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200
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GTX25621 WB Image
WB analysis of 25 ug of A431 (lane 1), rat thyroid (lane 2) and mouse thyroid (lane 3) cell lysates using GTX25621 Thyroid Hormone Receptor alpha antibody.
Dilution : 1:500
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GTX25621 ICC/IF Image
ICC/IF analysis of U251 cells using GTX25621 Thyroid Hormone Receptor alpha antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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REFERENCE
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