GeneTex

  • Country / Location Selection

United States (US)

Titin antibody [T11]

Cat. No. GTX27034

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 T11

Isotype

 IgG2b

Application

 WB, IHC-Fr, EM

Reactivity

 Chicken
Package
100 μl ($399)
See all Titin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr 1:1,000
EM Assay dependent
Not tested in other applications.

Positive Control

skeletal muscle

Product Note

The antibody localizes titin (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. The antibody does not cross-react with nebulin. Likewise, it does not react with smooth muscle, non-muscle tissues, or cultured cells.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

titin/nebulin fraction from chicken breast muscle.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

titin

Background

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting using total extracts of chicken breast muscle the antibody reacts specifically with both bands of the titin doublet, and showed no reaction with nebulin.

Database

Research Area

REFERENCE

There are currently no references for Titin antibody [T11] (GTX27034). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Titin antibody [T11] (GTX27034). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399