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Tp63 antibody

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in Immunohistochemistry (IHC). GTX124660
Anti-Tp63 antibody used in Western Blot (WB). GTX124660
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

Cat. No. GTX124660

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC-Wm, ChIP assay, IHC

Reactivity

 Southern platyfish, Zebrafish, Fish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish p63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
ChIP assay Assay dependent
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

zebrafish muscle

Predict Reactivity

Human (100%)| Rat (99%)| Zebrafish (94%)| Bovine (100%)| Chicken (96%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Tp63

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175363

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p63 , DNp63 , fc89f04 , id:ibd3516 , p63 , tp73l , wu:fc89f04 , wu:fk85h07 , wu:fk88b02

Database

Research Area

DATA IMAGES

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tp63 antibody (GTX124660) dilution: 1:100.
Anti-Tp63 antibody used in Immunohistochemistry (IHC). GTX124660

GTX124660 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using tp63 (GTX124660) antibody at 1:500 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-Tp63 antibody used in Western Blot (WB). GTX124660

GTX124660 WB Image

p63 antibody detects p63 protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX124660) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp63 antibody (GTX124660) dilution: 1:200.
Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

Tp63 antibody detects Tp63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Tp63 stained by Tp63 antibody (GTX124660) diluted at 1:100.

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

The data was published in the journal Nat Commun in 2019. PMID: 30837472

Anti-Tp63 antibody used in IHC (Whole mount) (IHC-Wm). GTX124660

GTX124660 IHC-Wm Image

The data was published in the 2019 in Front Mol Neurosci. PMID: 31616250

REFERENCE

Application Reference

Rees L et al. Front Cell Dev Biol 2023; 11 (0) : 1134451 Regeneration of the dermal skeleton and wound epidermis formation depend on BMP signaling in the caudal fin of platyfish.
Application : IHC-Fr
Reactivity : Southern platyfish
Sebastian Wurster et al. Cell Rep 2021; 34 (12) : 108896 EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion.
Application : IHC
Reactivity : Zebrafish
Fadul J et al. Nat Commun 2021; 12 (1) : 7180 KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion.
Application : IHC-Wm
Reactivity : Zebrafish
Brock CK et al. Nat Commun 2019; 10 (1) : 1044 Stem cell proliferation is induced by apoptotic bodies from dying cells during epithelial tissue maintenance.
K?nig D et al. Front Mol Neurosci 2019; 12 : 227 Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish.
Application : IHC-Wm
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REVIEW

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Application Reference
Rees L et al. Front Cell Dev Biol 2023; 11 (0):1134451 Regeneration of the dermal skeleton and wound epidermis formation depend on BMP signaling in the caudal fin of platyfish.
Application : IHC-Fr
Reactivity : Southern platyfish
Sebastian Wurster et al. Cell Rep 2021; 34 (12):108896 EGF-mediated suppression of cell extrusion during mucosal damage attenuates opportunistic fungal invasion.
Application : IHC
Reactivity : Zebrafish
Fadul J et al. Nat Commun 2021; 12 (1):7180 KRas-transformed epithelia cells invade and partially dedifferentiate by basal cell extrusion.
Application : IHC-Wm
Reactivity : Zebrafish
Brock CK et al. Nat Commun 2019; 10 (1):1044 Stem cell proliferation is induced by apoptotic bodies from dying cells during epithelial tissue maintenance.
K?nig D et al. Front Mol Neurosci 2019; 12:227 Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish.
Application : IHC-Wm
Bloomquist RF et al. Proc Natl Acad Sci U S A 2019; 116 (36):17858-17866 Developmental plasticity of epithelial stem cells in tooth and taste bud renewal.
Application : IHC-P
Reactivity : Fish
D?sir?e K?nig et al. bioRxiv 2019; (Epub) Distribution and restoration of serotonin-immunoreactive paraneuronal cells during caudal fin regeneration in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Santos-Pereira JM et al. Nat Commun 2019; 10 (1):3049 Pioneer and repressive functions of p63 during zebrafish embryonic ectoderm specification.
Application : IHC-Wm, ChIP assay
Reactivity : Zebrafish
Schepis A et al. J Cell Biol 2018; 217 (3):1097-1112 Protease signaling regulates apical cell extrusion, cell contacts, and proliferation in epithelia.
Kimelman D et al. Elife 2017; 6 Regulation of posterior body and epidermal morphogenesis in Zebrafish by localized Yap1 and Wwtr1.
Application : IHC-Fr
Reactivity : Zebrafish
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.
Application : IHC-P
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169