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Transglutaminase 2 antibody [N3C3]

KO/KD Orthogonal
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

Cat. No. GTX111702

Reference 6
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Transglutaminase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:26956429).

Predict Reactivity

Bovine, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transglutaminase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952227

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transglutaminase 2 , TG(C) , TGC

Background

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

GTX111702 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Transglutaminase 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111702

GTX111702 ICC/IF Image

Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transglutaminase 2 protein stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702

GTX111702 IHC-P Image

Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702

GTX111702 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using Transglutaminase 2(GTX111702) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702

GTX111702 IHC-P Image

Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Transglutaminase 2 stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transglutaminase 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111702

GTX111702 IHC-P Image

Transglutaminase 2 antibody [N3C3] detects Transglutaminase 2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Transglutaminase 2 stained by Transglutaminase 2 antibody [N3C3] (GTX111702) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

GTX111702 WB Image

Orthogonal

The data was published in the journal Elife in 2016.PMID: 26956429

Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

GTX111702 WB Image

KO/KD

The data was published in the journal Elife in 2016.PMID: 26956429

Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

GTX111702 WB Image

KO/KD Orthogonal

The data was published in the journal Elife in 2016.PMID: 26956429

Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

GTX111702 WB Image

Orthogonal

The data was published in the journal Elife in 2016.PMID: 26956429

Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702

GTX111702 WB Image

The data was published in the journal FEBS Open Bio in 2017. PMID: 28680807

REFERENCE

Application Reference

Cao Q et al. Matrix Biol 2022; 111 : 226-244 Collagen VII maintains proteostasis in dermal fibroblasts by scaffolding TANGO1 cargo.
Application : WB
Reactivity : Human
Xu P et al. Cell Metab 2022; 34 (2) : 299-316.e6 Erythrocyte transglutaminase-2 combats hypoxia and chronic kidney disease by promoting oxygen delivery and carnitine homeostasis.
Application : WB
Reactivity : Mouse
Morotomi-Yano K et al. FEBS Open Bio 2017; 7 (7) : 934-943 Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields.
Application : WB
Reactivity : Human
Morotomi-Yano K et al. FEBS Open Bio 2017; (Epub) Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields.
Application : WB
Reactivity : Human
Yeo SY et al. Elife 2016; 5 : e07101 Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Transglutaminase 2 antibody [N3C3] Cat. No. GTX111702

Rating
( Average 5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Transglutaminase 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111702
Date : Anonymous submitted on 20-Mar-2024
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 WT and TGM2 KO cells
Blocking : PBS, 5% BSA, 5% goat serum, 0.01% Triton X-100, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : PBS, 0.1% Triton X-100, 25°C, 0.17Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : WT cells: Green dashed line
KO cells: Magenta dashed line

Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Transglutaminase 2 antibody [N3C3] used in Immunoprecipitation (IP). GTX111702
Date : Anonymous submitted on 20-Mar-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : A549 cells
Lane Description : SM: 4% starting material
UB: 4% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.5 mL aliquots at 2.0 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC.

Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Date : Anonymous submitted on 20-Mar-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 WT and TGM2 KO cells
Amount used : 40μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Transglutaminase 2 antibody [N3C3] used in Western Blot (WB). GTX111702
Date : Anonymous submitted on 20-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OEC-M1 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169