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Twist1 antibody

Anti-Twist1 antibody used in Western Blot (WB). GTX50821
Anti-Twist1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50821

Cat. No. GTX50821

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Twist1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1: 500-1:1000
IHC-P 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.18~22(S-N-S-E-E) derived from human Twist1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163238

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

twist family bHLH transcription factor 1 , ACS3 , BPES2 , BPES3 , CRS , CRS1 , CSO , SCS , SWCOS , TWIST , bHLHa38

Cellular Localization

Nucleus

Background

This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Twist1 antibody used in Western Blot (WB). GTX50821

GTX50821 WB Image

WB analysis of extracts from JK, Raji, and HL-60 cells using GTX50821 Twist1 antibody.

Anti-Twist1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50821

GTX50821 IHC-P Image

IHC-P analysis of mouse pancreatic cancer tissue sections using GTX50821 Twist1 antibody.

REFERENCE

Application Reference

B Zeng et al. Int J Oncol 2024; 64 (4) : 45 Lysine methyltransferase 5C increases the proliferation and metastatic abilities of clear cell renal cell carcinoma via aerobic glycolysis
Application : WB
Jimenez-Garduno AM et al. Biochem Biophys Res Commun 2017; IL-1β induced methylation of the estrogen receptor ERα gene correlates with EMT and chemoresistance in breast cancer cells.
Application : WB
Reactivity : Human
Tsai CH et al. Biochim Biophys Acta 2015; 1852 (5) : 851-61 Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399