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UBE2I antibody

KO/KD
Anti-UBE2I antibody used in Western Blot (WB). GTX129293
Anti-UBE2I antibody used in IHC (Paraffin sections) (IHC-P). GTX129293
Anti-UBE2I antibody used in Western Blot (WB). GTX129293
Anti-UBE2I antibody used in Western Blot (WB). GTX129293

Cat. No. GTX129293

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UBE2I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse testis

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885959

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 I , C358B7.1 , P18 , UBC9

Cellular Localization

Nucleus

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE2I antibody used in Western Blot (WB). GTX129293

GTX129293 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2I antibody (GTX129293) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UBE2I antibody used in IHC (Paraffin sections) (IHC-P). GTX129293

GTX129293 IHC-P Image

UBC9 antibody detects UBC9 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
UBC9 antibody (GTX129293) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UBE2I antibody used in Western Blot (WB). GTX129293

GTX129293 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2I antibody (GTX129293) diluted at 1:1000.

Anti-UBE2I antibody used in Western Blot (WB). GTX129293

GTX129293 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2I antibody (GTX129293) diluted at 1:1000.

REFERENCE

Application Reference

Koh EH et al. J Biol Chem 2016; 291 (47) : 24747-24755 Mitochondrial Activity in Human White Adipocytes Is Regulated by the Ubiquitin Carrier Protein 9/microRNA-30a Axis.
Application : WB
Reactivity : Human
Tomasi ML et al. Free Radic Biol Med 2014; 68 : 148-58 Molecular mechanisms of lipopolysaccharide-mediated inhibition of glutathione synthesis in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169