APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , Jurkat , mouse testis , 293T
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2L3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10624592
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC , UBCH7 , UbcM4
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/templates/default/js/lightbox/images/loading.gif "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
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GTX114870 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114870 WB Image
Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000.
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GTX114870 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using UBE2L3 antibody [N1C3] (GTX114870) at 1:300 dilution.
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GTX114870 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000.
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GTX114870 WB Image
Sample (30 ug of whole cell lysate)
A: JurKat
B: Hela
12% SDS PAGE
GTX114870 diluted at 1:1000
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GTX114870 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using UBE2L3(GTX114870) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for UBE2L3 antibody [N1C3] (GTX114870). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UBE2L3 antibody [N1C3] (GTX114870). Be the first to share your experience with this product.
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/small/GTX114870/GTX114870_40233_20181221_WB_shRNA_watermark_w_23060519_791.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/small/GTX114870/GTX114870_40233_20160811_WB_Z_22111423_392.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114870](/upload/website/prouct_img/small/GTX114870/GTX114870_40233_IHC_Z_22111423_794.webp "Anti-UBE2L3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/small/GTX114870/GTX114870_40233_20160811_WB_M_testis_w_23060518_549.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/small/GTX114870/GTX114870_WB_w_23060518_227.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114870](/upload/website/prouct_img/small/GTX114870/GTX114870_IFA_w_23060519_158.webp "Anti-UBE2L3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/normal/GTX114870/GTX114870_40233_20181221_WB_shRNA_watermark_w_23060519_791.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/normal/GTX114870/GTX114870_40233_20160811_WB_Z_22111423_392.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114870](/upload/website/prouct_img/normal/GTX114870/GTX114870_40233_IHC_Z_22111423_794.webp "Anti-UBE2L3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/normal/GTX114870/GTX114870_40233_20160811_WB_M_testis_w_23060518_549.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870](/upload/website/prouct_img/normal/GTX114870/GTX114870_WB_w_23060518_227.webp "Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870")
![Anti-UBE2L3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114870](/upload/website/prouct_img/normal/GTX114870/GTX114870_IFA_w_23060519_158.webp "Anti-UBE2L3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114870")