APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse liver , Rat liver , UGDH-transfected 293T
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.36 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UGDH. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11165945
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
UDP-glucose 6-dehydrogenase , GDH , UDP-GlcDH , UDPGDH , UGD
Background
The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX104993 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UGDH antibody (GTX104993) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX104993 WB Image
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX104993 diluted at 1:10000
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GTX104993 WB Image
UGDH antibody detects UGDH protein by Western blot analysis.
A. 50 μg Rat liver lysate/extract
7.5 % SDS-PAGE
UGDH antibody (GTX104993) dilution: 1:2000
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GTX104993 ICC/IF Image
UGDH antibody detects UGDH protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UGDH stained by UGDH antibody (GTX104993) diluted at 1:500.
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GTX104993 IHC-P Image
UGDH antibody detects UGDH protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
UGDH antibody (GTX104993) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104993 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UGDH antibody (GTX104993) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for UGDH antibody (GTX104993). Be the first to share your publications with this product.
REVIEW
UGDH antibody
Cat. No. GTX104993
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 24-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
TOV-21G cells
Amount used :
60 μg
Blocking :
5 % milk, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
