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UNC119 antibody [N2C3]

Orthogonal
Anti-UNC119 antibody [N2C3] used in Western Blot (WB). GTX115185
Anti-UNC119 antibody [N2C3] used in Western Blot (WB). GTX115185
Anti-UNC119 antibody [N2C3] used in Western Blot (WB). GTX115185

Cat. No. GTX115185

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UNC119 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UNC119. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723081

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

unc-119 lipid binding chaperone , HRG4 , IMD13 , POC7 , POC7A

Background

This gene is specifically expressed in the photoreceptors in the retina. The encoded product shares strong homology with the C. elegans unc119 protein and it can functionally complement the C. elegans unc119 mutation. It has been localized to the photoreceptor synapses in the outer plexiform layer of the retina, and suggested to play a role in the mechanism of photoreceptor neurotransmitter release through the synaptic vesicle cycle. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UNC119 antibody [N2C3] used in Western Blot (WB). GTX115185

GTX115185 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115185 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UNC119 antibody [N2C3] used in Western Blot (WB). GTX115185

GTX115185 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2017.PMID: 28740133

Anti-UNC119 antibody [N2C3] used in Western Blot (WB). GTX115185

GTX115185 WB Image

Orthogonal

The data was published in the journal Nat Commun in 2017.PMID: 28740133

REFERENCE

Application Reference

Konitsiotis AD et al. Nat Commun 2017; 8 (1) : 114 Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.
Application : WB
Reactivity : Human
Lei B et al. Am J Cancer Res 2015; 5 (10) : 3123-34 Highly expressed UNC119 promotes hepatocellular carcinoma cell proliferation through Wnt/β-catenin signaling and predicts a poor prognosis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169