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Ubiquitin B antibody [3C12]

Anti-Ubiquitin B antibody [3C12] used in ELISA (ELISA). GTX60548
Anti-Ubiquitin B antibody [3C12] used in Western Blot (WB). GTX60548
Anti-Ubiquitin B antibody [3C12] used in Western Blot (WB). GTX60548
Anti-Ubiquitin B antibody [3C12] used in Flow cytometry (FACS). GTX60548

Cat. No. GTX60548

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3C12

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all Ubiquitin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human UBB expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin B , HEL-S-50

Cellular Localization

Nucleus

Background

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ubiquitin B antibody [3C12] used in ELISA (ELISA). GTX60548

GTX60548 ELISA Image

ELISA analysis of antigen using GTX60548 Ubiquitin B antibody [3C12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Ubiquitin B antibody [3C12] used in Western Blot (WB). GTX60548

GTX60548 WB Image

WB analysis of human Ubiquitin B (AA: 1-299) recombinant protein using GTX60548 Ubiquitin B antibody [3C12].

Anti-Ubiquitin B antibody [3C12] used in Western Blot (WB). GTX60548

GTX60548 WB Image

WB analysis of NIH3T3 (1) and HeLa (2) cell lysate using GTX60548 Ubiquitin B antibody [3C12].

Anti-Ubiquitin B antibody [3C12] used in Flow cytometry (FACS). GTX60548

GTX60548 FACS Image

FACS analysis of HeLa cells using GTX60548 Ubiquitin B antibody [3C12].
Green : Ubiquitin B
Red : negative control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409