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Ubiquitin antibody [GT7811]

Comparison Orthogonal Overexpression
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148
Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

Cat. No. GTX630148

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT7811

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Ubiquitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Positive Control

Myc-human Ubiquition-transfected 293T cells , 293T , A431 , HepG2 , HeLa (10 μM MG132 treatment for 16 hr) , Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Yeast, Drosophila, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Ubiquitin.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888189

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:5000.

Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148

GTX630148 IHC-P Image

Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in IHC (Paraffin sections) (IHC-P). GTX630148

GTX630148 IHC-P Image

Ubiquitin antibody [GT7811] detects Ubiquitin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Ubiquitin stained by Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Overexpression

Ubiquitin antibody detects Ubiquitin protein by western blot analysis. Non-transfected (-) and -transfected (+, including Myc-tag) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody (GTX630148) diluted by 1:10000.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Orthogonal

293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ubiquitin antibody [GT7811] used in Western Blot (WB). GTX630148

GTX630148 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Ubiquitin antibody [GT7811] (GTX630148) diluted at 1:500 and competitor's antibody (sc-53509) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Mhoriam Ahmed et al. Research Square 2023; Amplifying the heat shock response ameliorates ALS and FTD pathology in mouse and human models.
Application : ICC/IF, IHC-Fr
Reactivity : Mouse
Hsu WH et al. Int J Biol Macromol 2020; 165 (Pt A) : 1604-1613 Effects of WSG, a polysaccharide from Ganoderma lucidum, on suppressing cell growth and mobility of lung cancer.
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations