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Ubiquitin antibody [UBB/2122]

Anti-Ubiquitin antibody [UBB/2122] used in IHC (Paraffin sections) (IHC-P). GTX17824
Anti-Ubiquitin antibody [UBB/2122] used in Western Blot (WB). GTX17824
Anti-Ubiquitin antibody [UBB/2122] used in Protein Array (Protein Array). GTX17824
Anti-Ubiquitin antibody [UBB/2122] used in IHC (Paraffin sections) (IHC-P). GTX17824

Cat. No. GTX17824

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 UBB/2122

Isotype

 IgG1

Application

 WB, IHC-P, ELISA, Protein Array

Reactivity

 Species independent
Package
100 μg ($529)
See all Ubiquitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
ELISA Assay dependent
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Ubiquitin antibody [UBB/2122] used in IHC (Paraffin sections) (IHC-P). GTX17824

GTX17824 IHC-P Image

IHC-P analysis of human brain tissue using GTX17824 Ubiquitin antibody [UBB/2122].

Anti-Ubiquitin antibody [UBB/2122] used in Western Blot (WB). GTX17824

GTX17824 WB Image

WB analysis of Raji cell lysate using GTX17824 Ubiquitin antibody [UBB/2122].

Anti-Ubiquitin antibody [UBB/2122] used in Protein Array (Protein Array). GTX17824

GTX17824 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Ubiquitin Mouse Monoclonal Antibody (UBB/2122). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Ubiquitin antibody [UBB/2122] used in IHC (Paraffin sections) (IHC-P). GTX17824

GTX17824 IHC-P Image

IHC-P analysis of human brain tissue using GTX17824 Ubiquitin antibody [UBB/2122].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529