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VEGF antibody [VG76e]

Cat No. GTX20119

Host

Mouse

Clonality

Monoclonal

Clone Name

VG76e

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

Good results have been reported on human, bovine and porcine paraffin sections, but only after microwave treatment and using Tris HCl pH 10 buffer (NOT citrate buffer).VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length Human VEGF189 expressed in E.coli

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_373273

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

vascular endothelial growth factor A , MVCD1 , VEGF , VPF

Cellular Localization

Secreted

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Database

Research Area

REFERENCE

REVIEW

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