GeneTex
  • Country / Location Selection

United States (US)

VEGF antibody

Anti-VEGF antibody used in Western Blot (WB). GTX129844

Cat No. GTX129844

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HepG2 conditioned medium

Predict Reactivity

Mouse, Rat, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human VEGFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MVCD1 antibody , VEGF-A antibody , VEGFA antibody , VPF antibody , vascular endothelial growth factor A antibody

Cellular Localization

Secreted

Background

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-VEGF antibody used in Western Blot (WB). GTX129844

GTX129844 WB Image

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VEGF antibody (GTX129844) diluted at 1:500.
Multiple bands were observed 15~25 kDa. It is possibly due to alternative splicing.
Reference: PMID: 25732926 , PMID: 15896327 , PMID: 21283766

REFERENCE

There are currently no references for VEGF antibody (GTX129844). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for VEGF antibody (GTX129844). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf