VEGF121 recombinant protein

Cat No. GTX65275

Application	ELISA, Functional Assay, Apuri, Blocking
Reactivity	Human
Species	Human

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Datasheet

APPLICATION

Application Note

Protein's bioactivity determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 0.1-0.5 ng/ml.

Calculated MW

28.4 kDa. ( Note )

PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Human

Expression System

E. coli

Purification

Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

VEGFA, VPF, Vascular permeability factor, MGC70609., VEGF-A, VEGF, Vascular endothelial growth factor A, VEGF A

Background

VEGF (Vascular Endothelial Growth Factor) is a secreted homodimeric protein, secreted by a variety of Vascularized tissues. VEGF stimulates endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 28.4 kDa homodimeric protein consisting of two 121 amino acid polypeptide chains.