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VPS34 antibody

KO/KD Overexpression
Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Western Blot (WB). GTX129528
Anti-VPS34 antibody used in Immunoprecipitation (IP). GTX129528
Anti-VPS34 antibody used in Western Blot (WB). GTX129528

Cat. No. GTX129528

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all VPS34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IP 1:100-1:500

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , unboiled hVps34-transfected 293T cells

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human VPS34. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886028

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol 3-kinase catalytic subunit type 3 , VPS34 , Vps34 , hVps34

Background

Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.

Database

Research Area

DATA IMAGES

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody (GTX129528) diluted at 1:1000.

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

VPS34 antibody detects VPS34 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
VPS34 antibody (GTX129528) dilution: 1:1000

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

VPS34 antibody detects VPS34 protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
VPS34 antibody (GTX129528) dilution: 1:1000

Anti-VPS34 antibody used in Immunoprecipitation (IP). GTX129528

GTX129528 IP Image

Immunoprecipitation of VPS34 protein from HeLa whole cell extracts using 5 μg of VPS34 antibody (GTX129528).
Western blot analysis was performed using VPS34 antibody (GTX129528).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-VPS34 antibody used in Western Blot (WB). GTX129528

GTX129528 WB Image

Overexpression

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with VPS34 antibody (GTX129528) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liang YC et al. Cancers (Basel) 2020; 12 (6) The Antihistamine Deptropine Induces Hepatoma Cell Death through Blocking Autophagosome-Lysosome Fusion.
Chen CH et al. Cancers (Basel) 2019; 11 (9) Targeting Autophagy by MPT0L145, a Highly Potent PIK3C3 Inhibitor, Provides Synergistic Interaction to Targeted or Chemotherapeutic Agents in Cancer Cells.
Application : WB
Reactivity : Human
Jiang X et al. Oncogene 2017; 36 (50) : 6850-6862 VPS34 stimulation of p62 phosphorylation for cancer progression.
Wakatsuki S et al. J Cell Biol 2017; 216 (2) : 477-493 GSK3B-mediated phosphorylation of MCL1 regulates axonal autophagy to promote Wallerian degeneration.
Chen CH et al. Clin Cancer Res 2017; (Epub) Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169