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VRL1 antibody

Anti-VRL1 antibody used in Immunoprecipitation (IP). GTX16610
Anti-VRL1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16610
Anti-VRL1 antibody used in Western Blot (WB). GTX16610

Cat. No. GTX16610

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP

Reactivity

 Mouse, Rat
Package
50 μl ($639)
See all VRL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)KKNPTSKPGKNSASEE, corresponding to amino acid residues 735-750 ( Intracellular, C-terminus) of rat TRPV2 (Accession  : Q9WUD2).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel, subfamily V, member 2 , Vrl1

Cellular Localization

Cell membrane; Multi-pass membrane protein,Cytoplasm

Background

ion channel that may be activated by high temperature; may transduce high threshold heat responses in sensory ganglia [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-VRL1 antibody used in Immunoprecipitation (IP). GTX16610

GTX16610 IP Image

IP analysis of RBL cell lysate using GTX16610 VRL1 antibody. The upper arrow indicates TRPV2 while the lower arrow indicates the IgG heavy chain. WB was performed with the GTX16610 antibody.
IP reaction : 7.5 μg
Lane 1 : RBL lysate
Lane 2 : Lysate immunoprecipitated with Anti-TRPV2   Antibody
Lane 3 : Lysate immunoprecipitated with pre-immune rabbit serum

Anti-VRL1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX16610

GTX16610 IHC-Fr Image

IHC-Fr analysis of mouse DRG tissue using GTX16610 VRL1 antibody.
Panel A : TRPV2 (green) appears in patches along the perimeter of the DRG (arrows).
Panel B : Neurons containing neurofilament 200 (red) are scattered in the DRG, also in patches (arrows).
Panel C : Merge of the two panels shows that the spatial distribution of neurofilament 200 and TRPV2 expression overlaps. However, DRGs showing robust expression of neurofilament 200 do not contain TRPV2.

Anti-VRL1 antibody used in Western Blot (WB). GTX16610

GTX16610 WB Image

WB analysis of rat brain membrane (lanes 1 and 2) and RBL (lanes 3 and 4) lysates using GTX16610 VRL1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639