GeneTex
United States (US)

Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody

GTX64191_20170328_WB_18121411_841.jpg
GTX64191_20170328_IP_18121411_203.jpg

Cat No. GTX64191

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ELISA
Reactivity E. coli
APPLICATION

Application Note

Western blotting (2,000 fold dilution)
PROPERTIES

Form

Liquid

Buffer

Rabbit antiserum added with 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Initial immunization by VT1 toxoid and booster by VT1 toxin.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX64191_20170328_WB_18121411_841.jpg

GTX64191 WB Image

Detection of VT1 by western blotting with anti -VT1 antibody 1. SDS-PAGE of culture medium of VTEC, stained with CBB 2. SDS-PAGE of crude extracts of VTEC cells, stained with CBB 3. Western blotting of culture medium of VTEC 4. Western blotting of crude extracts of VTEC cells. Ani-VT1 antibody was used at 1/2,000 dilution

GTX64191_20170328_IP_18121411_203.jpg

GTX64191 IP Image

Immunoprecipitation of VT1 from culture medium of VTEC with anti-VT1 antibody Arrows shows subunit A and subunit B of VT1. Heavy chain and Light chain indicate those of IgG

Package List Price ($)
$ 349