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Vimentin antibody [4F2E9]

Anti-Vimentin antibody [4F2E9] used in IHC (Paraffin sections) (IHC-P). GTX83095
Anti-Vimentin antibody [4F2E9] used in Western Blot (WB). GTX83095

Cat. No. GTX83095

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F2E9

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Monkey
Package
100 μl ($409)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [4F2E9] used in IHC (Paraffin sections) (IHC-P). GTX83095

GTX83095 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX83095 Vimentin antibody [4F2E9].

Anti-Vimentin antibody [4F2E9] used in Western Blot (WB). GTX83095

GTX83095 WB Image

WB analysis of HeLa (1), COS (2), HEK293 (3) and U20S (4) cell lysate using GTX83095 Vimentin antibody [4F2E9].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409