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Vimentin antibody [VM452]

Anti-Vimentin antibody [VM452] used in Flow cytometry (FACS). GTX17723
Anti-Vimentin antibody [VM452] used in Western Blot (WB). GTX17723
Anti-Vimentin antibody [VM452] used in Western Blot (WB). GTX17723
Anti-Vimentin antibody [VM452] used in IHC (Paraffin sections) (IHC-P). GTX17723
Anti-Vimentin antibody [VM452] used in Protein Array (Protein Array). GTX17723

Cat. No. GTX17723

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VM452

Isotype

 IgG1

Application

 WB, IHC-P, FACS, Protein Array

Reactivity

 Human, Goat, Bovine, Cat, Dog, Chicken, Pig
Package
100 μg ($609)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human vimentin protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTRCT30 , HEL113 , VIM , vimentin , Vimentin

Cellular Localization

Cytoplasm

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Vimentin antibody [VM452] used in Flow cytometry (FACS). GTX17723

GTX17723 FACS Image

FACS analysis of Jurkat cells using GTX17723 Vimentin antibody [VM452].
Black : Blank
Grey : Isotype Control
Green : Primary antibody

Anti-Vimentin antibody [VM452] used in Western Blot (WB). GTX17723

GTX17723 WB Image

WB analysis of A375 cell lysate using GTX17723 Vimentin antibody [VM452].

Anti-Vimentin antibody [VM452] used in Western Blot (WB). GTX17723

GTX17723 WB Image

WB analysis of Raji cell lysate using GTX17723 Vimentin antibody [VM452].

Anti-Vimentin antibody [VM452] used in IHC (Paraffin sections) (IHC-P). GTX17723

GTX17723 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX17723 Vimentin antibody [VM452].

Anti-Vimentin antibody [VM452] used in Protein Array (Protein Array). GTX17723

GTX17723 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 609