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Vimentin (phospho Ser56) antibody

KO/KD Orthogonal
Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607
Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607
Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

Cat. No. GTX132607

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Vimentin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLa (100 ng/ml Nocodazole 24 hr) , 293T , NT2D1

Predict Reactivity

Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser56 of human Vimentin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886692

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

vimentin

Background

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

GTX132607 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (GTX132607) diluted at 1:5000.

Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

GTX132607 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (GTX132607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Vimentin (phospho Ser56) antibody used in Western Blot (WB). GTX132607

GTX132607 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin (phospho Ser56) antibody (GTX132607) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169