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Wnt10a antibody

Anti-Wnt10a antibody used in IHC (Paraffin sections) (IHC-P). GTX31345
Anti-Wnt10a antibody used in IHC (Paraffin sections) (IHC-P). GTX31345
Anti-Wnt10a antibody used in IHC (Paraffin sections) (IHC-P). GTX31345
Anti-Wnt10a antibody used in Western Blot (WB). GTX31345

Cat No. GTX31345

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

RAW 264.7 Cell Lysate(GTX27187)

Specificity/Sensitivity

This Wnt10a antibody is predicted to not cross-react with Wnt10b.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Wnt10a antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Wnt10a.The immunogen is located within amino acids 300 - 350 of Wnt10a.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wnt Family Member 10A,Oodd,Ssps,Sthag4,Wnt10A

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Wnt10a antibody used in IHC (Paraffin sections) (IHC-P). GTX31345

GTX31345 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX31345 WNT10A antibody.
Working concentration : 10 μg/ml

Anti-Wnt10a antibody used in IHC (Paraffin sections) (IHC-P). GTX31345

GTX31345 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX31345 WNT10A antibody.
Working concentration : 20 μg/ml

Anti-Wnt10a antibody used in IHC (Paraffin sections) (IHC-P). GTX31345

GTX31345 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX31345 WNT10A antibody.
Working concentration : 5 μg/ml

Anti-Wnt10a antibody used in Western Blot (WB). GTX31345

GTX31345 WB Image

WB analysis of RAW264.7 cell lysate using GTX31345 WNT10A antibody.
Working concentration : (A) 1 and (B) 2 μg/ml