GeneTex
United States (US)

Wnt4 antibody, Internal

GTX15699_73_ICC-IF_18121410_783.jpg
GTX15699_74_ICC-IF_18121410_442.jpg
GTX15699_1226_WB_18121410_389.jpg

Cat No. GTX15699

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF 10μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells.

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX15699-PEP).

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SNWLYLAKLSSVGS, from the internal region of the protein sequence according to NP_110388.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wnt Family Member 4,Serkal,Wnt-4,Wnt4

Cellular Localization

Possibly secreted and associates with the extracellular matrix.

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX15699_73_ICC-IF_18121410_783.jpg

GTX15699 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX15699 WNT4 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX15699_74_ICC-IF_18121410_442.jpg

GTX15699 ICC/IF Image

ICC/IF analysis of PFA fixed MCF7 cells using GTX15699 WNT4 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX15699_1226_WB_18121410_389.jpg

GTX15699 WB Image

WB analysis of Jurkat (A) and negative control U937 (B) cell lysate using GTX15699 WNT4 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer