GeneTex
United States (US)

WNT4 antibody

WNT4-antibody_WB_GTX30713-1_18121411_636.jpg

Cat No. GTX30713

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

For ELISA (peptide): Use at a dilution of 1:16,00-1:32,000. For WB: Use at a concentration of 0.03-0.1 μg/ml. A band at approximately 35kD is detected. Calculated MW of 39.1kD. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

39 kDa. ( Note )

Positive Control

Human testes and ovary
PROPERTIES

Form

Liquid

Buffer

Tris saline, pH 7.3 containing 0.5% BSA and 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 23-36 of Human WNT4

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

WNT4 is a ligand for members of the frizzled family of seven transmembrane receptors and a probable developmental protein. It may be a signalling molecule which affects the development of discrete regions of tissues and is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue.

Database

Research Area

DATA IMAGES
WNT4-antibody_WB_GTX30713-1_18121411_636.jpg

GTX30713 WB Image

Western blot analysis of WNT4 in a) human ovary and b) human testes lysate using GTX30713 at  0.03μg/ml