GeneTex
United States (US)

WNT5A antibody

GTX100618_40653_WB_B_19011506_473.jpg

Cat No. GTX100618

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

WNT5A transfected 293T lysates

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hWNT5A antibody, WNT5A antibody, protein Wnt-5a antibody, WNT-5A protein antibody, "wingless-type MMTV integration site family, member 5A antibody"

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100618_40653_WB_B_19011506_473.jpg

GTX100618 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: WNT5A transfected 293T lysates
10% SDS PAGE
GTX100618 diluted at 1:5000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf