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West Nile virus Envelope antibody

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

Cat No. GTX132052

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity West Nile virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

WNV-infected 293T , WNV-infected vero cell , WNV Envelope-transfected 293T cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of West Nile virus Envelope protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

WestNile virus Envelope protein antibody , WestNile virus (WNV) Envelopeprotein E antibody , WNV E antibody , WNV Envelope antibody , WNV E protein antibody

Research Area

DATA IMAGES
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

GTX132052 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody (GTX132052) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

GTX132052 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody (GTX132052).

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

GTX132052 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody (GTX132052) diluted at 1:5000.

REFERENCE
REVIEW

West Nile virus Envelope antibody Cat No. GTX132052

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:2000, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts.
Figure Note : Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf