GeneTex
United States (US)

West Nile virus Envelope protein antibody [GT3029]

GTX633919_42702_20180822_ICC_IF_B_18121411_156.jpg
GTX633919_42702_20170105_WB_B_18121411_457.jpg

Cat No. GTX633919

Host Mouse
Clonality Monoclonal
Clone Name GT3029
Isotype IgG1
Application WB, ICC/IF
Reactivity West Nile virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

West Nile virus Envelope protein E-transfected 293T
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

Recombinant protein encompassing a sequence within the center region of West Nile virus Envelope protein. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

West Nile virus Envelope antibody

Research Area

DATA IMAGES
GTX633919_42702_20180822_ICC_IF_B_18121411_156.jpg

GTX633919 ICC/IF Image

West Nile virus Envelope protein antibody [GT3029] detects West Nile virus Envelope protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: West Nile virus Envelope protein stained by West Nile virus Envelope protein antibody [GT3029] (GTX633919) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX633919_42702_20170105_WB_B_18121411_457.jpg

GTX633919 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody [GT3029] (GTX633919) diluted at 1:1000.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169